The Effect of Zn Nanoparticle Concentration and the Comparison of Growth Regulator Concentration on the Growth of Cavendish Banana Explants (Musa acuminata)
DOI:
https://doi.org/10.70063/eduspectrum.v1i2.46Keywords:
Cavendish Banan, Zn Nanoparticles, Growth RegulatorAbstract
Cavendish banana (Musa acuminata L.) propagation through conventional methods requires a considerable amount of time. Tissue culture techniques offer a more efficient alternative, enabling the production of plantlets with superior quality and quantity in a shorter period. The application of Zn nanoparticles has been shown to reduce media contamination and act as a biostimulant, enhancing propagation efficiency in tissue culture. Auxins, such as Naphthalene Acetic Acid (NAA), facilitate cell development and root induction, while cytokinins, including 6-Benzyl Amino Purine (BAP), are effective in stimulating cell division, morphogenesis, and shoot formation with improved stability and resistance to oxidation. This study was conducted from February to June 2023 at the Biotechnology Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional "Veteran" Jawa Timur. The research utilized a Completely Randomized Design (CRD) with two factors: the concentration of Zn nanoparticles and the ratio of ZPT concentrations. The results indicated that the best treatment for multiplication was obtained with 50 ppm Zn nanoparticles, resulting in the fastest shoot emergence at 24,63 days and the highest number of shoots per plantlet at 2,58 shoots. Furthermore, the treatment with a ZPT concentration ratio of NAA + BAP (0:0) produced the highest average number of plantlet leaves, with 5,78 leaves per plantlet.
Downloads
References
Ahmad, N., Alatar, A. A., Faisal, M., Khan, M. I., Fatima, N., Anis, M., & Hegazy, A. K. 2015. Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina. Biologia plantarum, 59, 11-17.
Avivi, S., Soedarmo, S. H., dan Prasetyo, P. A. 2013. Multiplikasi Tunas dan Aklimatisasi Tiga Varietas Pisang: Raja Nangka, Kepok, dan Mas. Jurnal Hortikultura Indonesia, 4 (2): 83-89.
Basha, S. A., dan Selvaraju, M. 2015. Toxic effect of Zinc on growth and nutrient accumulation of cow pea (Vigna unguiculata L.). International Letters of Natural Sciences, (43)
El-Mahdy, M. T., Radi, A. A., dan Shaaban, M. M. 2019. Impacts of Exposure of Banana to Silver Nanoparticles and Sliver Ions In Vitro. Middle East J Appl Sci, 9(3): 727-740.
Lala, S. 2021. Nanoparticles as elicitors and harvesters of economically important secondary metabolites in higher plants: A review. IET nanobiotechnology. 15(1): 28-57
Linda, R. 2018. Perbanyakan Tunas Pisang Cavendish (Musa acuminata) Secara In vitro dengan Penambahan Naphthalene Acetic Acid (NAA) dan Air Kelapa. Protobiont, 7 (1): 75-79.
Mandeh, M., M.Omidi, dan M. Rahaie. 2012. In vitro influences of tio2 nanoparticles on barley (Hordeum vulgare L.) Tissue Culture. BiologicalTrace Element Research 150 (1-3): 376-380.
Nadeak, R., Anna, N., & Siregar, E. B. M. 2012. Respon eskplan biji gaharu (Aquilaria malaccencis Lamk.) terhadap pemberian NAA dan IBA secara in vitro. Peronema Forestry Science Journal, 1(1), 156169.
Nisak, K, Nurhidayati, T & Kristanti IP, 2012, ‘Pengaruh Kombinasi Konsentrasi Zpt NAA dan BAP pada Kultur Jaringan Tembakau Nicotiana tabacum Var. prancak 95, Jurnal Sains dan Seni Pomits,vol 1 no. 1 hal. 1-6
Paserang, A. P., & Riska, R. 2022. Aplikasi Hormon BAP, NAA, Air Kelapa Terhadap Multiplikasi Pisang Cavendish (Musa Acuminata L.) Secara In Vitro. Biocelebes, 16(1), 38-46.
Sharma, A., Devadas, V. S., Sharma, H., Kartha, D., Pandey, H., Soni, G.,& Kumari, N. 2021. Advancement of agricultural technology in farming of india. Bhartiya Krishi Anusandhan Patrika, 36(4), 313-319.
Sintha, D., Atra, R., dan Widodo, W. 2017. Pengaruh BAP dan Kinetin Terhadap Pertumbuhan Tunas Pisang Barangan (Musa Paradisiaca L.) Secara In vitro. Doctoral Dissertation. Universitas Bengkulu.
Trinopsagiarti, T., & Seprido, S. 2021. Uji Berbagai Sitokinin Pada Media Ms Terhadap Pertumbuhan Globular Eksplan Pisang Barangan (Musa acuminata). Green Swarnadwipa: Jurnal Pengembangan Ilmu Pertanian, 10(2), 176-184.
